In recent years, ovarian revitalization methods have been applied worldwide in order to improve the hormonal and reproductive conditions of the patient and thus their quality of life. In the literature, the most prevalent is PLRP method of rejuvenation. Growth factors are found in the granules and they play an important role in cell proliferation, chemotaxis, promotion of angiogenesis and differentiation of mesenchymal and other types of cells.

Control mechanisms of cellular growth mainly consist of different growth factors. These factors are obtained from the patient’s own blood. In the ovaries, they control the production and growth of egg cells and the surrounding cells responsible for hormone production.​

Main characteristics of Ovarian PLRP (platelet-rich plasma) rejuvenation are explained here in more detail.

Plasma is a serum which contains thrombocytes and lymphocytes, coagulation factors and proteins. Thrombocytes are anuclear (nucleus-free) but contain around one thousand signal proteins, small granules (alfa, delta, and lambda). In 50 to 80 alfa granules per thrombocyte, there are almost 30 types of growth factors present (FR) connected with hemostasis, wound healing and repairing.

In PRP the thrombocyte concentration is 5 to 8 times higher than in physiological plasma. Thrombocyte response is important for the initiation of wound repair. Besides the coagulative effect, they are an important source of growth factors, such as platelet-derived growth factor (PDGF), transforming growth factor-b (TGF-b) 1 and 2, and vascular endothelial growth factor (VEGF). Adding calcium chloride and thrombin to PRP automatically activates alfa granules. This activation leads to the release of the above mentioned biological growth factors, PDGF, TGF-b, VEGF, insulin-like growth factor I, epidermal growth factor (EGF) and epithelial cell growth factor.

A number of studies have shown the important role of leucocytes in PRP. On one side, they are important for fighting infection, and on the other side, for immunoregulation. Leucocytes produce big amounts of VEGF. Considering that thrombocytes are rich in VEGF stimulators, additional VEGF of leucocyte origin can be crucial for angiogenesis.

The specificity of the SEGOVA-PLRP
Different technologies for the separation of cells from blood as well as the activation of growth factors provide varied results. The majority of systems for the separation of growth factor rich cells are based on specially prepared vials containing blood samples. The blood samples are then put in a centrifuge at a specific rate of rotation for a given time. This usually increases the concentration of cells up to 2 or 3 times.

In the SEGOVA PLRP process, special systems and machines for the separation of specific cell lines are used, which allows for the increase of the concentration of the desired cells (and therefore of the growth factors gained from them) up to 18 times the initial concentration. This allows us to choose the appropriate concentration for each individual patient, which is usually about 7 or 8 times more than the initial concentration.

Why is this important?
The effect of the growth factor depends on the concentration of it in the active substance introduced back into the patient. The use of sophisticated technology, similar to that used for the separation of stem cells, allows for a significantly higher concentration and effect of PLRP as part of the SEGOVA program.