SEGOVA REJUVENATION Program, created by ac. prof. Aleksandar Ljubic, is the most advanced, innovative and safest autologous method of rejuvenation of the gonads (ovaries and testes) with the use of patients own cells and growth factors to re-establish and enhance gonadal function and hormone production, thus improving reproductive health and whole-body functioning. 

The main advantage of SEGOVA is based on autologous regeneration of the gonads, the organs which change the most through ageing. Gonads, ovaries and testes, influence the functioning and structure of all other tissues and organs within the organism.

SEGOVA rejuvenation program, is a completely autologous program that avoids chemicals and medicaments. Hence, it is autologous rejuvenation program it is safe and without side effects. During the program, our Segova team creates personalized nutrition and physical activity programs for patients aimed at supporting gonads in creating the elements necessary to produce new egg cells and proper function of the existing endocrine-hormone producing cells.


Age-related ovarian dysfunction and fertility decline in women has emerged as an indication for ovarian rejuvenation as the age at which women now attempt their first pregnancy has been steadily rising over the last decades. Female fertility naturally decreases significantly after the age of 35 years, causing poor oocyte quality, abnormalities in the meiotic spindle, chromosome misalignment and shortened telomeres and poor quality of embryos.

For various reasons, women want to preserve fertility by programs of ovarian rejuvenation. Society today and the currents of modern civilization are such that they are forcing reproduction to be delayed for later years. Also, numerous diseases can significantly affect the decline in the ovarian function and quality of egg cells.


Segova treatment is highly beneficial in:
– Premature ovarian failure (POF syndrome)
– Unsuccessful IVF cycles in relation to maternal age
– Early-onset of menopause

Following the SEGOVA rejuvenation program, restored ovarian tissue takes over the role that it had before and begins the production of its own sex hormones. The lost functions of the ovaries are re-established and all other organs and tissues in the body are rejuvenated and their function is improved.


SEGOVA Program consists of several separate procedures integrated into a common project aimed to support, increase or create elements necessary for normal gonadal (ovaries or testes) function.

The first letters of the SEGOVA abbreviation in fact represent the specific procedures:

S – Stem cell therapy
Cell therapy which uses autologous stem cells – cells obtained from the patients’ own body. The aim is to create new or incite existing non-differentiated gonadal stem cells – which are to be developed into new oocytes or sperm cells and the hormone-producing cells.

E – Energy mitochondrial boosting
Modern biotechnological procedures that use stem cell and substrates derived from them – exosomes, can influence the quantity and quality of mitochondria. Boosting mitochondrial energy production in ovarian cells can be done also with a specific physical exercise regime – HIIT (High Intensity Interval Training). HIIT is a combination of highly intensive, intermittent, specific anaerobic exercise.

G – Growth factor PLPR therapy
Growth factors are essential mediators in gonadal control mechanisms. These growth factors are obtained from the patient’s own blood and used to treat gonadal tissue. They act by controlling the creation and growth of eggs and the surrounding cells responsible for hormone production. In recent years, ovarian rejuvenation methods have been applied worldwide in order to improve the hormonal and reproductive conditions of the patient and thus their quality of life. PLRP is a separated part of the whole blood where the high platelet level is concentrated, but with growth factors of 3 to 5 times the plasma concentration. Growth factors are found in the granules and they play an important role in cell proliferation, chemotaxis, promotion of angiogenesis and differentiation of mesenchymal and other types of cells.

OVA – Ovarian in Vitro Activation
The production of eggs – oogenesis, is dependent on proper genetic control. Hippo the signalling pathway is essential for maintaining optimum organ size. It contains several negative growth regulators. The AKT the signalling pathway has a key role in the initiation of follicle growth. The ovarian in vitro activation represents the autologous genetic treatment of the gonadal tissue in order to restore both reproductive and endocrine functions of the ovary.

Program in Detail


  • Preparation – one or more days or weeks, including analyses of:

hormones, immunological status, infections, metabolism, vitamins, microelements and genetics.

  • Performing of Procedure – during the one-day stay in the hospital,

Blood is drawn from the patient to isolate and concentrate autologous PRP; BRF and autologous thrombin (fully automatised closed system); General anaesthesia; Biopsy of the iliac bone and aspiration of the bone marrow; Separation and processing of mSC from the bone marrow aspirate concentrate (fully automatised closed system); Minimally invasive laparoscopic surgery with tangential ovarian cortex excision; Administration of autologous haemostatics instead of classical destructive surgical techniques; Micro-fragmentation of ovarian tissue submerged in mediums with autologous activated PRP – IVA; Creation of subcortical tunnels for the IVA ovarian cortical tissue and mSC retransplantation; Application of autologous activated BRF scaffold in the adequate subcortical tunnels; Application of the micro fragmented IVA ovarian cortical tissue with mSC into the adequate subcortical spaces; Closure of the tunnels and re-creating the ovarian surface with the activated autologous BRF; Control of haemostasis

Discharge from the hospital in 1 or 2 days.

  • The additional activities – the Nutritionist for Metabolic Optimisation and the physiotherapist – Gonadal HIIT program.
  • Follow-up – monthly or annual ultrasound and laboratory follow-up, with suggestions or implementation of the advanced in vitro fertilisation techniques.

Read more


SEGOVA program for men is a safe, innovative method, based on autologous, organic regeneration of the testes. It requires the use of patients own cells and growth factors to re-establish and enhance testicular function and hormone production, thus improving reproductive health and whole-body functioning (skin, face, muscles, bones, blood vessels and other). It helps to delay andropause and prevents age-related diseases.

The age-related decline in testosterone along with the associated symptoms has been referred to as male menopause or the andropause. Partial androgen deficiency and the decline in testosterone seen in ageing men has clinical implications. Along with the decline in testosterone, some men experience symptoms that include: fatigue, weakness, depression, sexual dysfunction. Standard medical therapy today – Testosterone replacement is connected with potential risks and side effects.

SEGOVA Program for men follows the standard set of SEGOVA procedures and includes the standard follow up program.

Follow up program

SEGOVA Program follow up begins at the end of the SEGOVA program completion. We provide test results upon your departure and following the first month of the program we ask you to perform certain blood work tests and share the results with us. Usually the same, sometimes additional, tests are then performed at quarterly intervals during the period of up to two years if not otherwise requested by SEGOVA medical team and agreed with the patient.