SEGOVA REJUVENATION Program, created by ac. prof. Aleksandar Ljubic, is the most advanced, innovative and safest autologous method of rejuvenation of the gonads (ovaries and testes) with the use of patients own cells and growth factors to re-establish and enhance gonadal function and hormone production, thus improving reproductive health and whole-body functioning. 

The main advantage of SEGOVA is based on autologous regeneration of the gonads, the organs which change the most through ageing. Gonads, ovaries and testes, influence the functioning and structure of all other tissues and organs within the organism.

SEGOVA rejuvenation program, is a completely autologous program that avoids chemicals and medicaments. Hence, it is autologous rejuvenation program it is safe and without side effects. During the program, our Segova team creates personalized nutrition and physical activity programs for patients aimed at supporting gonads in creating the elements necessary to produce new egg cells and proper function of the existing endocrine-hormone producing cells.


Age-related ovarian dysfunction and fertility decline in women has emerged as an indication for ovarian rejuvenation as the age at which women now attempt their first pregnancy has been steadily rising over the last decades. Female fertility naturally decreases significantly after the age of 35 years, causing poor oocyte quality, abnormalities in the meiotic spindle, chromosome misalignment and shortened telomeres and poor quality of embryos.

For various reasons, women want to preserve fertility by programs of ovarian rejuvenation. Society today and the currents of modern civilization are such that they are forcing reproduction to be delayed for later years. Also, numerous diseases can significantly affect the decline in the ovarian function and quality of egg cells.

Segova treatment is highly beneficial in:
– Premature ovarian failure (POF syndrome)
– Unsuccessful IVF cycles in relation to maternal age
– Early-onset of menopause

Following the SEGOVA rejuvenation program, restored ovarian tissue takes over the role that it had before and begins the production of its own sex hormones. The lost functions of the ovaries are re-established and all other organs and tissues in the body are rejuvenated and their function is improved.


SEGOVA Program consists of several separate procedures integrated into a common project aimed to support, increase or create elements necessary for normal gonadal (ovaries or testes) function.

The first letters of the SEGOVA abbreviation in fact represent the specific procedures:

S – Stem cell therapy
Cell therapy which uses autologous stem cells – cells obtained from the patients’ own body. The aim is to create new or incite existing non-differentiated gonadal stem cells – which are to be developed into new oocytes or sperm cells and the hormone-producing cells.

E – Energy mitochondrial boosting
Modern biotechnological procedures that use stem cell and substrates derived from them – exosomes, can influence the quantity and quality of mitochondria. Boosting mitochondrial energy production in ovarian cells can be done also with a specific physical exercise regime – HIIT (High Intensity Interval Training). HIIT is a combination of highly intensive, intermittent, specific anaerobic exercise.

G – Growth factor PLPR therapy
Growth factors are essential mediators in gonadal control mechanisms. These growth factors are obtained from the patient’s own blood and used to treat gonadal tissue. They act by controlling the creation and growth of eggs and the surrounding cells responsible for hormone production. In recent years, ovarian rejuvenation methods have been applied worldwide in order to improve the hormonal and reproductive conditions of the patient and thus their quality of life. PLRP is a separated part of the whole blood where the high platelet level is concentrated, but with growth factors of 3 to 5 times the plasma concentration. Growth factors are found in the granules and they play an important role in cell proliferation, chemotaxis, promotion of angiogenesis and differentiation of mesenchymal and other types of cells.

OVA – Ovarian in Vitro Activation
The production of eggs – oogenesis, is dependent on proper genetic control. Hippo the signalling pathway is essential for maintaining optimum organ size. It contains several negative growth regulators. The AKT the signalling pathway has a key role in the initiation of follicle growth. The ovarian in vitro activation represents the autologous genetic treatment of the gonadal tissue in order to restore both reproductive and endocrine functions of the ovary.

segova new generation

SEGOVA New Generation (NG) is the innovative, most complete available medical biological programme for the rejuvenation and improvement of ovarian function. SEGOVA NG implies a shortened procedure, which is possible due to the application of specific operative techniques during tissue retransplantation and the introduction of novel scaffolds.

Active principles:

  1. hcPRP – Activated autologous highly concentrated platelet growth factors (hcPRP)
  2. BRF – Activated autologous bio-regenerative fibrin (BRF)
  3. BM mSC – Mesenchymal bone marrow stem cells (bm mSC)
  4. IVA – In Vitro Activated (IVA) ovarian tissue
  5. Metabolic and physical optimisation – Patient’s participation in metabolic optimization and specific training


  • Preparation: One or more days or weeks, including analyses of hormones, immunological status, infections, metabolism, vitamins, microelements and genetics.
  • Performing of SEGOVA NG Procedure: The one-day stay in the hospital, general anaesthesia; Autologous activated hcPRP; BRF; mSC; One whole laparoscopic surgery; IVA – Micro-fragmentation of ovarian tissue; BRF scaffold in the adequate subcortical tunnels; BRF Neocortex; Discharge from the hospital in 1 or 2 days.
  • The additional activities:the Nutritionist for Metabolic Optimisation and the physiotherapist – Gonadal HIIT program.
  • Reproductive Follow-up: Regular ultrasound and laboratory follow-up, with suggestions or implementation of advanced in vitro fertilisation techniques.
SEGOVA Boosting spectrum

SEGOVA Boosting Spectrum (SEGOVA BS) is a set of procedures created to enhance and extend the positive effects of Segova. The application of SEGOVA BS is justified in cases where there is a positive response to SEGOVA.

These technologies have been developed for patients who have responded positively to SEGOVA, but pregnancy has not occurred. Additionally, it is indicated for patients in whom the positive effects of SEGOVA have started to diminish.

Considering the significant individual differences in response to biological therapies, continuous reproductive and endocrine monitoring after the procedure and the choice of therapy are of great importance.

Our primary intention is to capitalize on every opportunity to obtain eggs and embryos. To this end, supportive therapies have been developed that, based on individual characteristics, could increase the chance of achieving one’s own offspring.

Follow up program

SEGOVA Program follow up begins at the end of the SEGOVA program completion. We provide test results upon your departure and following the first month of the program we ask you to perform certain blood work tests and share the results with us. Usually the same, sometimes additional, tests are then performed at quarterly intervals during the period of up to two years if not otherwise requested by SEGOVA medical team and agreed with the patient.